A high recovery method for concentrating microgram quantities of protein from large volumes of solution

A method is presented for the recovery of 40–80% of the protein from a 1 μg/ml solution. The final protein pellet is free of detergent and other ionic compounds and is thus compatible with any denaturing solution. The primary structure of the protein is unaffected by the procedure, making the final...

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Veröffentlicht in:	Analytical biochemistry 1983-03, Vol.129 (2), p.513-516
Hauptverfasser:	Mahuran, D., Clements, P., Carrella, M., Strasberg, P.M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Chemical Precipitation Detergents Electrophoresis, Polyacrylamide Gel Isoelectric Focusing Microchemistry microtechniques, sodium dodecyl sulfate-polyacrylamide gel electrophoresis peptide mapping protein concentration Protein Denaturation protein precipitation Proteins - isolation & purification Sodium Dodecyl Sulfate Solutions
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Beschreibung
Zusammenfassung:	A method is presented for the recovery of 40–80% of the protein from a 1 μg/ml solution. The final protein pellet is free of detergent and other ionic compounds and is thus compatible with any denaturing solution. The primary structure of the protein is unaffected by the procedure, making the final pellet an ideal sample for any analytical procedure to determine protein structure.
ISSN:	0003-2697 1096-0309
DOI:	10.1016/0003-2697(83)90585-7