Steroid binding to synaptic plasma membrane: Differential binding of glucocorticoids and gonadal steroids

The specific binding of [ 3H]-corticosterone, [ 3H]-17β-estradiol, [ 3H]-testosterone, and [ 3H]-progesterone to synaptic plasma membrane (SPM) prepared from rat brain has been characterized. The dissociation constant is estimated as on the order of 1 × 10 −7 M for corticosterone and 1 × 10 −8 M for the other three steroids. In a competition experiment, none of the 3H-steroids was displaced by the other steroids at 500-fold excess, indicating the presence of specific binding sites on the membrane for each type of steroid. Moreover, pre-incubation of the SPM with phospholipase A 2 or phospholipase C totally destroys the membrane binding of corticosterone and testosterone, but the binding of estradiol and progesterone remains intact. Since the SPM prepared from brain tissue is derived from many different neuronal cell types, it is possible that the membrane binding sites for glucocorticoids and for gonadal steroids are present in different neurons.