Assessment of cerebrospinal fluid immunoglobulin patterns after isoelectric focusing: Use of kappa and lambda light chain immunoperoxidase staining

Assessment of the presence of immunoglobulin (Ig) of restricted heterogeneity i.c. oligoclonal Ig in cerebrospinal fluid subjected to isoelectric focusing (IEF) may be difficult because of the tendency of the technique to subdivide even normal polyclonal IgG into bands and zones. Focused Ig stained by the immunoperoxidase technique for kappa and lambda light chains shows correspondence between the two patterns in the case of normal polyclonal Ig, but a marked discrepancy between kappa and lambda patterns in disorders associated with oligoclonal Ig, and kappa and lambda immunofixation makes assessment of agarose IEF separations of IgG more reliable.