Vitronectin in the substratum of endothelial cells is cross-linked and phosphorylated

Vitronectin (VN), previously shown to be a substrate for purified transglutaminases, was demonstrated in this study to be cross-linked when incubated with HUVEC and EAhy926 cells. The cross-linking was calcium-dependent and required that VN be plated at the substratum of the cells. These cells also...

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Veröffentlicht in:Biochemical and biophysical research communications 1991-01, Vol.174 (2), p.465-469
Hauptverfasser: Sane, David C., Moser, Tammy L., Greenberg, Charles S.
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Sprache:eng
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Zusammenfassung:Vitronectin (VN), previously shown to be a substrate for purified transglutaminases, was demonstrated in this study to be cross-linked when incubated with HUVEC and EAhy926 cells. The cross-linking was calcium-dependent and required that VN be plated at the substratum of the cells. These cells also phosphorylated VN, but in contrast to a previous study demonstrating a cAMP-dependent protein kinase in platelets, the phosphorylation of VN by was decreased with the addition of 1mM cAMP. The cross-linking of VN by endothelial cells demonstrates that the adhesion of these cells to VN is a dynamic process in which the substratum may be enzymatically altered. Furthermore, the modifications of VN by cross-linking and phosphorylation could modulate the functions of VN and influence events such as endothelial cell proliferation and angiogenesis.
ISSN:0006-291X
1090-2104
DOI:10.1016/0006-291X(91)91439-J