Stereospecific, reversible binding of D-[ 3H] glucose to crude membrane fractions prepared from rat brain

Stereospecific, reversible binding of D-[ 3H] glucose to crude membrane fractions prepared from rat brain

To a crude preparation of synaptic membranes prepared from rat brain, stereospecific, saturable, reversible binding was described of D-[ 3H]glucose. Binding showed a K d = 0.45 μM and the fractional rate of dissociation was approximately eight times the fractional rate of association. D-[ 3H]glucose...

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Veröffentlicht in:Life sciences (1973) 1983-04, Vol.32 (16), p.1865-1872
Hauptverfasser: Gold, Barry I., Kyle-Lillegard, Jayne
Format: Artikel
Sprache:eng
Schlagworte:
3-O-Methylglucose
Animals
Brain - metabolism
Deoxyglucose - metabolism
Glucose - metabolism
Hot Temperature
Kinetics
Male
Membrane Proteins - metabolism
Methylglucosides - metabolism
Protein Denaturation
Rats
Rats, Inbred Strains
Synaptic Membranes - metabolism
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Zusammenfassung:To a crude preparation of synaptic membranes prepared from rat brain, stereospecific, saturable, reversible binding was described of D-[ 3H]glucose. Binding showed a K d = 0.45 μM and the fractional rate of dissociation was approximately eight times the fractional rate of association. D-[ 3H]glucose binding was displaced by 2-deoxyglucose and 3-0-methylglucose and it was abolished when membranes were denatured by heating.
ISSN:0024-3205
1879-0631
DOI:10.1016/0024-3205(83)90065-6