Bile acid uptake and calcium flux in brush border membrane vesicles

Our laboratory has recently reported that intestinal bile acid malabsorption in cystic fibrosis (CF) is a primary mucosal cell defect. Others have suggested that elevated intracellular Ca ++ levels in other cell types in CF may represent a common primary dysfunction in Ca ++ efflux in these cells. We examined the possibility that intestinal bile acid absorption and Ca ++ efflux in mucosal cells may be linked physiologically. Brush border membrane vesicles (BBMV) prepared from guinea pig ileum served as the experimental model to test this hypothesis. Ca ++ (2.5×10 −3M) present in the incubation medium did not alter the uptake of taurocholic acid (TCA) by BBMV. Also, TCA uptake into BBMV preloaded with Ca ++ was not significantly different from that in BBMV not previously loaded with Ca ++. Furthermore, with TCA present in the incubation medium, Ca ++ efflux from preloaded BBMV was not altered. These data suggest that ileal TCA uptake, as measured by BBMV, is not dependent upon either intra- or extravesicular Ca ++. Also, Ca ++ efflux from BBMV is unaffected by TCA uptake. Although separate lines of evidence suggest that intestinal bile acid malabsorption and reduced plasma membrane Ca ++ flux are primary defects in CF, we conclude that in the normal intestine these functions are independent physiological processes.