Methods and mechanisms for the development of MTX resistance in human lymphoid cells

Methods and mechanisms for the development of MTX resistance in human lymphoid cells

Conditions are described for the development of methotrexate resistance in CCRF/CEM human lymphoid cells and an attempt is made to distinguish between those conditions that lead to methotrexate resistance through impairment of cellular transport of methotrexate and those that lead to resistance thro...

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Veröffentlicht in:International journal of cancer 1983-01, Vol.31 (1), p.45-50
Hauptverfasser: Alam, A., Geddes, A. J., North, A. C. T.
Format: Artikel
Sprache:eng
Schlagworte:
Biological Transport
Cell Line
Drug Resistance
Female
Folic Acid Antagonists
Humans
In Vitro Techniques
Lymphocytes - drug effects
Methotrexate - pharmacology
Tetrahydrofolate Dehydrogenase - metabolism
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container_title International journal of cancer
container_volume 31
creator Alam, A.
Geddes, A. J.
North, A. C. T.
description Conditions are described for the development of methotrexate resistance in CCRF/CEM human lymphoid cells and an attempt is made to distinguish between those conditions that lead to methotrexate resistance through impairment of cellular transport of methotrexate and those that lead to resistance through enhanced production of dihydrofolate reductase. A restistant line showing a 200‐fold enhancement of dihydrofolate reductase has been obtained. The CCRF/CEM human cells are much more sensitive to methotrexate than are murine cells.
doi_str_mv 10.1002/ijc.2910310108
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source Wiley-Blackwell Journals; MEDLINE
subjects Biological Transport
Cell Line
Drug Resistance
Female
Folic Acid Antagonists
Humans
In Vitro Techniques
Lymphocytes - drug effects
Methotrexate - pharmacology
Tetrahydrofolate Dehydrogenase - metabolism
title Methods and mechanisms for the development of MTX resistance in human lymphoid cells
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