Methods and mechanisms for the development of MTX resistance in human lymphoid cells

Conditions are described for the development of methotrexate resistance in CCRF/CEM human lymphoid cells and an attempt is made to distinguish between those conditions that lead to methotrexate resistance through impairment of cellular transport of methotrexate and those that lead to resistance thro...

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Veröffentlicht in:	International journal of cancer 1983-01, Vol.31 (1), p.45-50
Hauptverfasser:	Alam, A., Geddes, A. J., North, A. C. T.
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological Transport Cell Line Drug Resistance Female Folic Acid Antagonists Humans In Vitro Techniques Lymphocytes - drug effects Methotrexate - pharmacology Tetrahydrofolate Dehydrogenase - metabolism
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Zusammenfassung:	Conditions are described for the development of methotrexate resistance in CCRF/CEM human lymphoid cells and an attempt is made to distinguish between those conditions that lead to methotrexate resistance through impairment of cellular transport of methotrexate and those that lead to resistance through enhanced production of dihydrofolate reductase. A restistant line showing a 200‐fold enhancement of dihydrofolate reductase has been obtained. The CCRF/CEM human cells are much more sensitive to methotrexate than are murine cells.
ISSN:	0020-7136 1097-0215
DOI:	10.1002/ijc.2910310108