Structure and expression of a cloned cDNA for human interleukin-2

A cDNA coding for human interleukin-2 (IL-2) has been cloned from a cDNA library prepared from partially purified IL-2 mRNA. The DNA sequence codes for a polypeptide which consists of 153 amino acids including a putative signal sequence. A biologically active polypeptide, characteristic of human IL-...

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Veröffentlicht in:	Nature (London) 1983-01, Vol.302 (5906), p.305-310
Hauptverfasser:	Taniguchi, Tadatsugu, Matsui, Hiroshi, Fujita, Takashi, Takaoka, Chikako, Kashima, Nobukazu, Yoshimoto, Ryota, Hamuro, Junji
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Animals Base Sequence Cells, Cultured Cloning, Molecular DNA - genetics Gene Expression Regulation Haplorhini Humanities and Social Sciences Humans Interleukin-2 - genetics multidisciplinary Operon Plasmids Science Science (multidisciplinary)
Online-Zugang:	Volltext
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Beschreibung
Zusammenfassung:	A cDNA coding for human interleukin-2 (IL-2) has been cloned from a cDNA library prepared from partially purified IL-2 mRNA. The DNA sequence codes for a polypeptide which consists of 153 amino acids including a putative signal sequence. A biologically active polypeptide, characteristic of human IL-2, was produced when the cDNA was fused to a simian virus 40 promoter sequence and used to transfect cultured monkey COS cells.
ISSN:	0028-0836 1476-4687
DOI:	10.1038/302305a0