A rapid method for detecting oligoclonal IgG in unconcentrated CSF, by agarose isoelectric focusing, transfer to cellulose nitrate and immunoperoxidase staining
A method has been developed for determining the electrophoretic distribution of IgG in unconcentrated cerebrospinal fluid by agarose isoelectric focusing, followed by transfer of focused protein from the agarose on to cellulose nitrate membranes. A double antibody immunoperoxidase staining technique...
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| Veröffentlicht in: | Journal of neuroimmunology 1983-01, Vol.4 (2), p.141-148 |
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| container_end_page | 148 |
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| container_issue | 2 |
| container_start_page | 141 |
| container_title | Journal of neuroimmunology |
| container_volume | 4 |
| creator | Walker, R.W.H. Keir, G. Johnson, M.H. Thompson, E.J. |
| description | A method has been developed for determining the electrophoretic distribution of IgG in unconcentrated cerebrospinal fluid by agarose isoelectric focusing, followed by transfer of focused protein from the agarose on to cellulose nitrate membranes. A double antibody immunoperoxidase staining technique is used, which is both rapid and sensitive. |
| doi_str_mv | 10.1016/0165-5728(83)90018-8 |
| format | Article |
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A double antibody immunoperoxidase staining technique is used, which is both rapid and sensitive.</description><subject>Agarose isoelectric focusing</subject><subject>Cellulose</subject><subject>Cellulose nitrate</subject><subject>Cerebrospinal fluid</subject><subject>Humans</subject><subject>IgG</subject><subject>Immunoenzyme Techniques</subject><subject>Immunoglobulin G - cerebrospinal fluid</subject><subject>Immunoperoxidase staining</subject><subject>Isoelectric Focusing</subject><subject>Nitrates</subject><subject>Sepharose</subject><issn>0165-5728</issn><issn>1872-8421</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1983</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkVFrHCEUhaW0pJtt_0ELPpUGMqmOOjovhbA0aSCQh7TP4uqdrWVGt-qU5t_0p8bJLnlsH0ThfPdcOQehd5RcUEK7T_WIRshWfVTsrCeEqka9QCuqZNso3tKXaPWMvEanOf-sjGC8P0EnHWO9EHKF_l7iZPbe4QnKj-jwEBN2UMAWH3Y4jn4X7RiDGfHN7hr7gOdgY7AQSjIFHN7cX53j7QM2O5NiBuxzhLFOJ2-rl51ztTnHFQ55gIRLxBbGcR4XNvgnE2yCw36a5hD3kOIf70wVczE-1OE36NVgxgxvj_cafb_68m3ztbm9u77ZXN42lilVmq1zzBLghHGmjOy2xgomiaSiU45zJVrXUSbZMDAOshW9am0_2K4fZC8sNWyNPhx89yn-miEXPfm8_NUEiHPWinBKe87_C1LW0bpvAfkBtDWZnGDQ--Qnkx40JXppUC_16KUerZh-arA-1uj90X_eTuCeh46VVf3zQYeaxm8PSWfroVbifKrBaxf9vxc8AlNYrQk</recordid><startdate>19830101</startdate><enddate>19830101</enddate><creator>Walker, R.W.H.</creator><creator>Keir, G.</creator><creator>Johnson, M.H.</creator><creator>Thompson, E.J.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7TK</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19830101</creationdate><title>A rapid method for detecting oligoclonal IgG in unconcentrated CSF, by agarose isoelectric focusing, transfer to cellulose nitrate and immunoperoxidase staining</title><author>Walker, R.W.H. ; Keir, G. ; Johnson, M.H. ; Thompson, E.J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c388t-bdd3c0e403438a76bac537071568d44852d61373ff34e725982c9fc69f795c1a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1983</creationdate><topic>Agarose isoelectric focusing</topic><topic>Cellulose</topic><topic>Cellulose nitrate</topic><topic>Cerebrospinal fluid</topic><topic>Humans</topic><topic>IgG</topic><topic>Immunoenzyme Techniques</topic><topic>Immunoglobulin G - cerebrospinal fluid</topic><topic>Immunoperoxidase staining</topic><topic>Isoelectric Focusing</topic><topic>Nitrates</topic><topic>Sepharose</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Walker, R.W.H.</creatorcontrib><creatorcontrib>Keir, G.</creatorcontrib><creatorcontrib>Johnson, M.H.</creatorcontrib><creatorcontrib>Thompson, E.J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of neuroimmunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Walker, R.W.H.</au><au>Keir, G.</au><au>Johnson, M.H.</au><au>Thompson, E.J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A rapid method for detecting oligoclonal IgG in unconcentrated CSF, by agarose isoelectric focusing, transfer to cellulose nitrate and immunoperoxidase staining</atitle><jtitle>Journal of neuroimmunology</jtitle><addtitle>J Neuroimmunol</addtitle><date>1983-01-01</date><risdate>1983</risdate><volume>4</volume><issue>2</issue><spage>141</spage><epage>148</epage><pages>141-148</pages><issn>0165-5728</issn><eissn>1872-8421</eissn><abstract>A method has been developed for determining the electrophoretic distribution of IgG in unconcentrated cerebrospinal fluid by agarose isoelectric focusing, followed by transfer of focused protein from the agarose on to cellulose nitrate membranes. 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| fulltext | fulltext |
| identifier | ISSN: 0165-5728 |
| ispartof | Journal of neuroimmunology, 1983-01, Vol.4 (2), p.141-148 |
| issn | 0165-5728 1872-8421 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_80411944 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Agarose isoelectric focusing Cellulose Cellulose nitrate Cerebrospinal fluid Humans IgG Immunoenzyme Techniques Immunoglobulin G - cerebrospinal fluid Immunoperoxidase staining Isoelectric Focusing Nitrates Sepharose |
| title | A rapid method for detecting oligoclonal IgG in unconcentrated CSF, by agarose isoelectric focusing, transfer to cellulose nitrate and immunoperoxidase staining |
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