A rapid method for detecting oligoclonal IgG in unconcentrated CSF, by agarose isoelectric focusing, transfer to cellulose nitrate and immunoperoxidase staining

A rapid method for detecting oligoclonal IgG in unconcentrated CSF, by agarose isoelectric focusing, transfer to cellulose nitrate and immunoperoxidase staining

A method has been developed for determining the electrophoretic distribution of IgG in unconcentrated cerebrospinal fluid by agarose isoelectric focusing, followed by transfer of focused protein from the agarose on to cellulose nitrate membranes. A double antibody immunoperoxidase staining technique...

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Veröffentlicht in:Journal of neuroimmunology 1983-01, Vol.4 (2), p.141-148
Hauptverfasser: Walker, R.W.H., Keir, G., Johnson, M.H., Thompson, E.J.
Format: Artikel
Sprache:eng
Schlagworte:
Agarose isoelectric focusing
Cellulose
Cellulose nitrate
Cerebrospinal fluid
Humans
IgG
Immunoenzyme Techniques
Immunoglobulin G - cerebrospinal fluid
Immunoperoxidase staining
Isoelectric Focusing
Nitrates
Sepharose
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Zusammenfassung:A method has been developed for determining the electrophoretic distribution of IgG in unconcentrated cerebrospinal fluid by agarose isoelectric focusing, followed by transfer of focused protein from the agarose on to cellulose nitrate membranes. A double antibody immunoperoxidase staining technique is used, which is both rapid and sensitive.
ISSN:0165-5728
1872-8421
DOI:10.1016/0165-5728(83)90018-8