H1 histone kinases from nuclei of Physarum polycephalum

Nuclear H1 histone kinase activity from Physarum plasmodia was separated into three major components by DEAE-cellulose chromatography. The enzyme fractions were termed kinase R, kinase A, and kinase B and were characterized by investigating (i) their dependence on cAMP, (ii) the effect of the heat-s...

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Veröffentlicht in:	Biochemistry (Easton) 1983, Vol.22 (1), p.30-37
Hauptverfasser:	Chambers, Timothy C, Langan, Thomas A, Matthews, Harry R, Bradbury, E. Morton
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Cattle Cell Nucleus - enzymology Chromatography, DEAE-Cellulose Chymotrypsin - metabolism Cyclic AMP - pharmacology fungi Isoenzymes - isolation & purification Peptide Fragments - analysis Phosphorylation Physarum - genetics Physarum polycephalum Protamine Kinase - isolation & purification Protein Kinases - isolation & purification Thymus Gland - analysis
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Zusammenfassung:	Nuclear H1 histone kinase activity from Physarum plasmodia was separated into three major components by DEAE-cellulose chromatography. The enzyme fractions were termed kinase R, kinase A, and kinase B and were characterized by investigating (i) their dependence on cAMP, (ii) the effect of the heat-stable inhibitor of cAMP-dependent protein kinase, (iii) the sites in calf H1 histone phosphorylated by each kinase, and (iv) the location of incorporation of phosphate in Physarum H1 histone catalyzed by each kinase in vitro.
ISSN:	0006-2960 1520-4995
DOI:	10.1021/bi00270a005