Partial purification of a high affinity taurine binding protein by affinity chromatography

Taurine (2-aminoethanesulfonic acid) is a ubiquitous amino acid found in very high concentrations in mammalian tissues. Recently, high affinity taurine binding sites were identified, detergent solubilized, and shown to have affinities similar to the beta -amino acid transport system. In this communication, the authors present for the first time an affinity chromatographic method for the purification of a high affinity taurine transport protein from cardiac sarcolemma.