Comparative studies on thermostability of calmodulin, skeletal muscle troponin C and their tryptic fragments

To obtain additional information about the effect of Ca super(2+) and Mg super(2+) on the secondary structure of Ca super(2+)-binding proteins, the authors studied thermostability of skeletal muscle troponin C and calmodulin using circular dichroism technique and the influence of Ca super(2+)-and Mg super(2+)-binding on thermal unfolding. Tryptic fragments of both calcium-binding proteins were used to localise the structural changes caused by temperature changes in the particular parts of both molecules.