Rifamycin B. oxidase from Monocillium spp., a new type of diphenol oxidase

It was found that enzyme from a microbial strain, Monocillium spp. ATCC 20621, catalyzed the oxidative reaction of rifamycin B to form rifamycin O. The identification of the reaction products suggested that the reaction proceeded by the oxidative cyclization of rifamycin B to give rifamycin O, which...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	FEBS letters 1983-01, Vol.151 (1), p.36-40
Hauptverfasser:	Han, Moon H., Baik-Lin, Seong, Son, Hyeung-Jin, Tae-Ick, Mheen
Format:	Artikel
Sprache:	eng
Schlagworte:	Biotransformation Antibiotic Chromatography Enzyme Laccase Mitosporic Fungi - enzymology Monocillium NAD - pharmacology NADP - pharmacology Oxidoreductases - isolation & purification Oxidoreductases - metabolism Polyphenol oxidase Rifamycin Rifamycin B oxidase Rifamycins - metabolism Substrate Specificity
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	It was found that enzyme from a microbial strain, Monocillium spp. ATCC 20621, catalyzed the oxidative reaction of rifamycin B to form rifamycin O. The identification of the reaction products suggested that the reaction proceeded by the oxidative cyclization of rifamycin B to give rifamycin O, which spontaneously hydrolyzed to rifamycin S in neutral aqueous milieu. The characteristic of the enzyme was different as compared with that of other polyphenol oxidases such as laccase. It is proposed that this new type of enzyme be classified into a subgroup EC 1.10.3.6 with a trival name rifamycin B oxidase.
ISSN:	0014-5793 1873-3468
DOI:	10.1016/0014-5793(83)80337-8