Molecular investigations on the high-affinity nerve growth factor receptor

Nerve growth factor (NGF) receptors have been investigated by means of affinity labeling with 1251-NGF and chemical cross-linking. Two distinct NGF-receptor complexes are detected on PC 12 cells; these correspond to 100 kd and 158 kd for the low-affinity (LNGFR) and the high-affinity (HNGFR) receptors, respectively. interestingly, three different antibodies directed against distinct epitopes on the LNG FIR immunoprecipitate the low- but not the high-affinity NGF-receptor complex. Although the identities of the signaling molecules in the HNGFR are unknown, antibodies to the src, ras, raf-1, and yes products fail to immunoprecipitate either receptor complex, suggesting that these molecules are not a part of, or tightly coupled to, either receptor type. Phosphotyrosine residues are found exclusively on the HNGFR complex, suggesting that tyrosine phosphorylation may be one of the initiating events in the NGF-induced signal transduction cascade.