The primary B cell response to the O/core region of bacterial lipopolysaccharide is restricted to the Ly-1 lineage

Experiments were performed to test the hypothesis that Ly-1 B cells respond to antigenic challenge with LPS from gram-negative bacteria. To perform these experiments, the splenic fragment culture system for the study of B cell precursors was used. We found that a significant number of anti-O/core, b...

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Veröffentlicht in:The Journal of immunology (1950) 1991-01, Vol.146 (1), p.327-331
Hauptverfasser: Su, SD, Ward, MM, Apicella, MA, Ward, RE
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Sprache:eng
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Zusammenfassung:Experiments were performed to test the hypothesis that Ly-1 B cells respond to antigenic challenge with LPS from gram-negative bacteria. To perform these experiments, the splenic fragment culture system for the study of B cell precursors was used. We found that a significant number of anti-O/core, but not anti-lipid A, precursors expressed the lambda L chain. A restriction of the anti-LPS response to Ly-1 B cells was tested using a Ly-1 depletion protocol. We found that the anti-O/core antibody response was restricted to the Ly-1 B cell lineage. In contrast, conventional B cells, not Ly-1 B cells, respond to an antigenic challenge with lipid A. Our results further support the idea that the Ly-1 B cell lineage serves a direct role in protecting against certain bacterial infections.
ISSN:0022-1767
1550-6606
DOI:10.4049/jimmunol.146.1.327