Highly sensitive determination of PLP in human plasma with HPLC method

To investigate the validity of using HPLC procedure with the fluorescence detection for the estimation of the pyridoxal 5'-phosphate (PLP) concentration in human blood plasma, we modified the conditions of our previous HPLC method for PLP. By changing the pH of the mobile phase (pH 5.5) and by...

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Veröffentlicht in:	Journal of Nutritional Science and Vitaminology 1990, Vol.36(6), pp.521-529
Hauptverfasser:	Hirose, N. (Gifu Univ. (Japan). Faculty of Agriculture), Kubo, N, Tsuge, H
Format:	Artikel
Sprache:	eng
Schlagworte:	Adolescent Adult ANALYTICAL METHODS BLOOD PLASMA CHROMATOGRAPHIE LIQUIDE HAUTE PRESS Chromatography, High Pressure Liquid - methods CROMATOGRAFIA LIQUIDA ALTA PRESION Female Fluorescence FOSFOLIPIDOS GENERO HUMANO GENRE HUMAIN HIGH PRESSURE LIQUID CHROMATOGRAPHY HPLC analysis of pyridoxal 5'-phosphate Humans Hydrogen-Ion Concentration KCN treatment of pyridoxal 5'-phosphate Male MANKIND Middle Aged PHOSPHATIDE PHOSPHOLIPIDS PIRIDOXINA PLASMA SANGUIN PLASMA SANGUINEO pyridoxal 5'-phosphate contents in human plasma Pyridoxal Phosphate - blood pyridoxic acid 5'-phosphate PYRIDOXINE Pyridoxine - blood Sensitivity and Specificity TECHNIQUE ANALYTIQUE TECNICAS ANALITICAS vitamin B6 analysis in human plasma by HPLC
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Zusammenfassung:	To investigate the validity of using HPLC procedure with the fluorescence detection for the estimation of the pyridoxal 5'-phosphate (PLP) concentration in human blood plasma, we modified the conditions of our previous HPLC method for PLP. By changing the pH of the mobile phase (pH 5.5) and by altering the detection wavelengths in fluorescence (excitation at 322nm and emission at 417nm), both the improved sensitivity for pyridoxic acid 5'-phosphate and elimination of interfering extraneous peaks were attained. We reported the total vitamin B6 content of healthy adult volunteers obtained by this HPLC method.
ISSN:	0301-4800 1881-7742
DOI:	10.3177/jnsv.36.521