A radioactive assay for the physiological activity of the tryptophan synthetase α subunit in crude extracts of Escherichia coli

A modified assay has been devised for the physiological reaction, indole-3-glycerol phosphate to Trp, of the enzyme tryptophan synthetase. The assay may be applied to crude bacterial extracts, and is based on the measurement of incorporation of radioactivity from [ 3H]Ser into Trp. Comparison with p...

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Veröffentlicht in:	Biochimie 1990-12, Vol.72 (12), p.873-879
Hauptverfasser:	Sörensen, P.M., Hijazi, K.A., Murgola, E.J., Buckingham, R.H.
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Biological and medical sciences Colorimetry Enzymes and enzyme inhibitors Escherichia coli - enzymology Escherichia coli - genetics Evaluation Studies as Topic Fundamental and applied biological sciences. Psychology Lyases Mutation Protein Conformation radioactive assay Tritium Tryptophan Synthase - analysis Tryptophan Synthase - chemistry Tryptophan Synthase - genetics tryptophan synthetase
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Beschreibung
Zusammenfassung:	A modified assay has been devised for the physiological reaction, indole-3-glycerol phosphate to Trp, of the enzyme tryptophan synthetase. The assay may be applied to crude bacterial extracts, and is based on the measurement of incorporation of radioactivity from [ 3H]Ser into Trp. Comparison with previous colorimetric assays indicates an improvement in sensitivity of about 30-fold, and advantages in terms of sample economy and simplified manipulation.
ISSN:	0300-9084 1638-6183
DOI:	10.1016/0300-9084(90)90005-2