Quantitative separation of estrogens, androgens and progestogens using reverse phase high pressure liquid chromatography
Dual UV wavelength scanning at 206 and 254 nm was used to develop a sensitive, separation and quantitation procedure for estrone, estradiol-17β, testosterone, 4-androstene-3,17,-dione, progesterone and 17-hydroxyprogesterone using reverse phase high pressure liquid chromatography. The difference in...
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| Veröffentlicht in: | Steroids 1982-10, Vol.40 (4), p.381-388 |
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| container_title | Steroids |
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| creator | Kling, O.Ray diZerega, Gere S. |
| description | Dual UV wavelength scanning at 206 and 254 nm was used to develop a sensitive, separation and quantitation procedure for estrone, estradiol-17β, testosterone, 4-androstene-3,17,-dione, progesterone and 17-hydroxyprogesterone using reverse phase high pressure liquid chromatography. The difference in UV absorption of the estrogens from the androgens and progestogens allows for correction of the co-elution of testosterone with estradiol-17β and 4-androsten-3,17,-dione with estrone. Incorporation of an isocratic step-gradient provides improved separation while maintaining shortened elution times for the less polar steroids. |
| doi_str_mv | 10.1016/0039-128X(82)90015-0 |
| format | Article |
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The difference in UV absorption of the estrogens from the androgens and progestogens allows for correction of the co-elution of testosterone with estradiol-17β and 4-androsten-3,17,-dione with estrone. 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The difference in UV absorption of the estrogens from the androgens and progestogens allows for correction of the co-elution of testosterone with estradiol-17β and 4-androsten-3,17,-dione with estrone. Incorporation of an isocratic step-gradient provides improved separation while maintaining shortened elution times for the less polar steroids.</description><subject>17-alpha-Hydroxyprogesterone</subject><subject>Androgens - blood</subject><subject>Androstenedione - blood</subject><subject>Animals</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Estradiol - blood</subject><subject>Estrogens - blood</subject><subject>Estrone - blood</subject><subject>Hydroxyprogesterones - blood</subject><subject>Progesterone - blood</subject><subject>Progestins - blood</subject><subject>Spectrophotometry, Ultraviolet - methods</subject><subject>Testosterone - blood</subject><issn>0039-128X</issn><issn>1878-5867</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1982</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE9r4zAQxcWypZum-w1a0GlpoW5HtmVLl4Wl9B8ESqGFvQlFGsdaEtuR7NB8-8rr0GMv0kPz3szoR8gZg2sGrLgByGTCUvH3QqSXEoDxBL6RGROlSLgoyu9k9mn5QU5C-AcARSbTY3JcshLKXM7I-8ugm971unc7pAE77aNsG9pWFEPv2xU24Yrqxk5yVLQbdeinhyG4ZkU97tAHpF2t41m7VR1dGMLgka7ddnCWmtq3Gx1TXnf1_pQcVXod8OfhnpO3-7vX28dk8fzwdPtnkZiMl31iEQpbiBR4jtxWiKxEwzWXqamMqbTE5VIUlkORS8t1VgimRc4sYznjQshsTn5NfePS2yFurTYuGFyvdYPtEJSALGWZzKIxn4zGtyF4rFTn3Ub7vWKgRuBqpKlGmkqk6j9wBTF2fug_LDdoP0MHwrH-e6pj_OTOoVfBOGwMWufR9Mq27usBH-Lpk30</recordid><startdate>198210</startdate><enddate>198210</enddate><creator>Kling, O.Ray</creator><creator>diZerega, Gere S.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198210</creationdate><title>Quantitative separation of estrogens, androgens and progestogens using reverse phase high pressure liquid chromatography</title><author>Kling, O.Ray ; diZerega, Gere S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-de06d682054e5dfee17ec5a592cfccfa9ebb86d50649d5a3681a841d114158893</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1982</creationdate><topic>17-alpha-Hydroxyprogesterone</topic><topic>Androgens - blood</topic><topic>Androstenedione - blood</topic><topic>Animals</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Estradiol - blood</topic><topic>Estrogens - blood</topic><topic>Estrone - blood</topic><topic>Hydroxyprogesterones - blood</topic><topic>Progesterone - blood</topic><topic>Progestins - blood</topic><topic>Spectrophotometry, Ultraviolet - methods</topic><topic>Testosterone - blood</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kling, O.Ray</creatorcontrib><creatorcontrib>diZerega, Gere S.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Steroids</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kling, O.Ray</au><au>diZerega, Gere S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Quantitative separation of estrogens, androgens and progestogens using reverse phase high pressure liquid chromatography</atitle><jtitle>Steroids</jtitle><addtitle>Steroids</addtitle><date>1982-10</date><risdate>1982</risdate><volume>40</volume><issue>4</issue><spage>381</spage><epage>388</epage><pages>381-388</pages><issn>0039-128X</issn><eissn>1878-5867</eissn><abstract>Dual UV wavelength scanning at 206 and 254 nm was used to develop a sensitive, separation and quantitation procedure for estrone, estradiol-17β, testosterone, 4-androstene-3,17,-dione, progesterone and 17-hydroxyprogesterone using reverse phase high pressure liquid chromatography. 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| fulltext | fulltext |
| identifier | ISSN: 0039-128X |
| ispartof | Steroids, 1982-10, Vol.40 (4), p.381-388 |
| issn | 0039-128X 1878-5867 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_80321393 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | 17-alpha-Hydroxyprogesterone Androgens - blood Androstenedione - blood Animals Chromatography, High Pressure Liquid - methods Estradiol - blood Estrogens - blood Estrone - blood Hydroxyprogesterones - blood Progesterone - blood Progestins - blood Spectrophotometry, Ultraviolet - methods Testosterone - blood |
| title | Quantitative separation of estrogens, androgens and progestogens using reverse phase high pressure liquid chromatography |
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