SYNTHESIS OF A HEPTACONTAPEPTIDE CORRESPONDING TO THE ENTIRE AMINO ACID SEQUENCE OF EGLIN C

A heptacontapeptide corresponding to the entire amino acid sequence of eglin c was synthesized by the conventional solution method using a minimal protecting method. The synthetic eglin c exhibited a symmetrical single peak on HPLC at the same retention time as an authetic eglin c, and had the same inhibitory activity against human leukocyte elastase, cathepsin G and α-chymotrypsin (Ki = 6.0 x 10-9 M, 5.5 x 10-9M and 2.5 x 10-9 M, respectively) as Nα-acetyl-eglin c synthesized genetically (Ki = 5.1 x 10-9 M, 1.5 x 10-8 M and 2.2 x 10-9 M, respectively).