Collagenase activity in the cervix of non-pregnant and pregnant women

Cervical biopsies were obtained from non pregnant patients and from pregnant at various stages of gestation and during labour. The tissues were extract with a Ca(++)-containing buffer, and collagenase activity was determined in these extracts using a solid phase assay in which triple helical 125I-la...

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Veröffentlicht in:	Archives of gynecology and obstetrics 1990-11, Vol.248 (2), p.75-80
Hauptverfasser:	Osmers, R, Rath, W, Adelmann-Grill, B C, Fittkow, C, Severényi, M, Kuhn, W
Format:	Artikel
Sprache:	eng
Schlagworte:	Abortion, Induced Cervix Uteri - enzymology Connective Tissue - enzymology Female Humans Labor Stage, First - physiology Labor Stage, Third - physiology Labor, Obstetric - physiology Microbial Collagenase - metabolism Pregnancy - physiology Pregnancy Trimester, First
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Beschreibung
Zusammenfassung:	Cervical biopsies were obtained from non pregnant patients and from pregnant at various stages of gestation and during labour. The tissues were extract with a Ca(++)-containing buffer, and collagenase activity was determined in these extracts using a solid phase assay in which triple helical 125I-labelled collagen was cleaved. Collagenase was detected in all samples but significantly elevated activity was only present in labour at 6-8 cm cervical dilatation. This provides direct evidence for the crucial role of specific collagen degradation during cervical ripening and dilatation.
ISSN:	0932-0067 1432-0711
DOI:	10.1007/bf02389578