Molecular characterization of non-histone chromatin proteins from experimental tumours

Using two-dimensional (2-D) electrophoresis, two non-histone chromatin protein fractions (NHCP1 and NHCP2) from three animal tumours (Kirkman-Robbins hepatoma, Morris hepatoma 7777 and Ehrlich ascites cells) and normal hamster liver were analyzed. Apart from many common components several tissue spe...

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Veröffentlicht in:	Acta biochimica polonica 1990, Vol.37 (2), p.267-276
Hauptverfasser:	Kiliańska, Z, Krajewska, W M, Lipińska, A, Marszałek, M, Gaczyński, M, Kłyszejko-Stefanowicz, L
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Carcinoma, Ehrlich Tumor - chemistry Cell Nucleus - chemistry chromatin Chromatin - chemistry Chromosomal Proteins, Non-Histone - chemistry Cricetinae Electrophoresis, Gel, Two-Dimensional Liver - chemistry Liver Neoplasms, Experimental - chemistry Mice Molecular Weight Neoplasms, Experimental - chemistry Rats Rosaniline Dyes Silver tumours
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Zusammenfassung:	Using two-dimensional (2-D) electrophoresis, two non-histone chromatin protein fractions (NHCP1 and NHCP2) from three animal tumours (Kirkman-Robbins hepatoma, Morris hepatoma 7777 and Ehrlich ascites cells) and normal hamster liver were analyzed. Apart from many common components several tissue specific polypeptides of the NHCP1 and NHCP2 fractions were detected. It was found that some spots present in electropherograms of non-histone proteins of tumour cells (M X 10(-3)/pI): 17-24/4.9-6.5 (NHCP1 and NHCP2); 34-41/4.9-6.0 (HCP1 and NHCP2); 44-46/5.3-7.5 (HCP2); 46-49/5.0-7.5 (NHCP1); 49/5.9-7.5 (NHCP2) and 102-134/5.6-7.0 (NHCP1) were absent from normal liver.
ISSN:	0001-527X