Structure of Polysaccharide-Peptidoglycan Complex from the Cell Wall of Lactobacillus casei YIT9018

The isolation and analysis of the polyeaccharide-peptidoglycan complexes of Lactobacillus casei YIT9018 are presented. Two polysaccharide-peptidoglycan complexes, PS-PG1 and PS-PG2, were solublized from the heat-killed cell by treatment with N-acetylmuramidase. PS-PG1 was composed of glucose, rhamno...

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Veröffentlicht in:	Journal of biochemistry (Tokyo) 1990-10, Vol.108 (4), p.568-571
Hauptverfasser:	Nagaoka, Masato, Muto, Masami, Nomoto, Koji, Matuzaki, Takesi, Watanabe, Tunekazu, Yokokura, Teruo
Format:	Artikel
Sprache:	eng
Schlagworte:	Carbohydrate Conformation Carbohydrate Sequence Cell Wall - chemistry Chromatography, Gel Deamination Lactobacillus - analysis Lactobacillus casei Molecular Sequence Data Peptidoglycan - chemistry Peptidoglycan - isolation & purification Polysaccharides - chemistry
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Zusammenfassung:	The isolation and analysis of the polyeaccharide-peptidoglycan complexes of Lactobacillus casei YIT9018 are presented. Two polysaccharide-peptidoglycan complexes, PS-PG1 and PS-PG2, were solublized from the heat-killed cell by treatment with N-acetylmuramidase. PS-PG1 was composed of glucose, rhamnose, and small amount of galactose and glucosamine. PS-PG2 was composed of glucose, rhamnose, galactosamine, and glucosamlne. The ratio by weight of these fractions was about 1 : 8. PS-PG2 was analyzed in detail. Smith degradation and deamination of this complex yielded oligosaccharide units. The results of methylation analysis of these units and intact PS-PG2 led to the most probable structure of PS-PG2:
ISSN:	0021-924X 1756-2651
DOI:	10.1093/oxfordjournals.jbchem.a123243