The development of beta-adrenergic receptors in the visual cortex of the rat
The development of beta-adrenergic receptors in the rat visual cortex was examined and the density of beta-receptors and associated subtypes (beta 1 and beta 2) was compared between visual and non-visual or whole cortical tissues using radioreceptor assays employing [ 125I]iodohydroxybenzylpindolol...
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Veröffentlicht in: | Neuroscience 1982-01, Vol.7 (11), p.2649-2655 |
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Sprache: | eng |
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Zusammenfassung: | The development of beta-adrenergic receptors in the rat visual cortex was examined and the density of beta-receptors and associated subtypes (beta
1 and beta
2) was compared between visual and non-visual or whole cortical tissues using radioreceptor assays employing [
125I]iodohydroxybenzylpindolol and [
125I]iodocyanopindolol as ligands. Saturation assays revealed not only similar affinities of beta-receptors for [
125I]iodohydroxybenzylpindolol in visual cortical samples at 10, 24 and 160 days after birth but also practically identical saturation curves for visual and non-visual cortical samples at 160 days of age. Displacement of [
125I]iodohydroxybenzylpindolol with propranolol in visual cortical membranes at various postnatal ages showed a gradual increase in receptor density from day 4 to day 24 with no change thereafter. No significant differences were observed in the overall density of betareceptors or in the distribution and density of beta
1 and beta
2-receptors between visual and non-visual or whole cortical samples; however, there was a definite decline in the density of beta-receptors in these samples between 40 and 160 days of age.
The results indicate that the developmental pattern of beta-receptor density and the distribution of beta
1 and beta
2-receptors are similar between visual and whole cortical tissues. In addition, the results emphasize the importance of maintaining the dissociation constant at a fixed value when comparing receptor densities between experiments, and also show the utility of employing the high-affinity ligand, [
125I]iodocyanopindolol, with a combination of serotoninergic, dopaminergic and alpha-adrenergic antagonists to examine beta-adrenergic receptors in a specific region of the brain. Study of betareceptors in the visual cortex may be beneficial in elucidating the role of norepinephrine in this region. |
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ISSN: | 0306-4522 1873-7544 |
DOI: | 10.1016/0306-4522(82)90089-6 |