The development of beta-adrenergic receptors in the visual cortex of the rat

The development of beta-adrenergic receptors in the rat visual cortex was examined and the density of beta-receptors and associated subtypes (beta 1 and beta 2) was compared between visual and non-visual or whole cortical tissues using radioreceptor assays employing [ 125I]iodohydroxybenzylpindolol...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Neuroscience 1982-01, Vol.7 (11), p.2649-2655
Hauptverfasser: McDonald, J.K., Petrovic, S.L., McCann, S.M., Parnavelas, J.G.
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:The development of beta-adrenergic receptors in the rat visual cortex was examined and the density of beta-receptors and associated subtypes (beta 1 and beta 2) was compared between visual and non-visual or whole cortical tissues using radioreceptor assays employing [ 125I]iodohydroxybenzylpindolol and [ 125I]iodocyanopindolol as ligands. Saturation assays revealed not only similar affinities of beta-receptors for [ 125I]iodohydroxybenzylpindolol in visual cortical samples at 10, 24 and 160 days after birth but also practically identical saturation curves for visual and non-visual cortical samples at 160 days of age. Displacement of [ 125I]iodohydroxybenzylpindolol with propranolol in visual cortical membranes at various postnatal ages showed a gradual increase in receptor density from day 4 to day 24 with no change thereafter. No significant differences were observed in the overall density of betareceptors or in the distribution and density of beta 1 and beta 2-receptors between visual and non-visual or whole cortical samples; however, there was a definite decline in the density of beta-receptors in these samples between 40 and 160 days of age. The results indicate that the developmental pattern of beta-receptor density and the distribution of beta 1 and beta 2-receptors are similar between visual and whole cortical tissues. In addition, the results emphasize the importance of maintaining the dissociation constant at a fixed value when comparing receptor densities between experiments, and also show the utility of employing the high-affinity ligand, [ 125I]iodocyanopindolol, with a combination of serotoninergic, dopaminergic and alpha-adrenergic antagonists to examine beta-adrenergic receptors in a specific region of the brain. Study of betareceptors in the visual cortex may be beneficial in elucidating the role of norepinephrine in this region.
ISSN:0306-4522
1873-7544
DOI:10.1016/0306-4522(82)90089-6