An automated assay for the determination of asparaginase activity

Accurate and precise determinations of activity are required during the production of the drug asparaginase. Experience in a control laboratory situation has shown that manual enzymatic activity assays have a high degree of variability between chemists because of slight differences in technique. An...

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Veröffentlicht in:Journal of biological standardization 1982-01, Vol.10 (4), p.297-301
Hauptverfasser: Toncic, Pauline F., Tibbetts, Donald L.
Format: Artikel
Sprache:eng
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Zusammenfassung:Accurate and precise determinations of activity are required during the production of the drug asparaginase. Experience in a control laboratory situation has shown that manual enzymatic activity assays have a high degree of variability between chemists because of slight differences in technique. An automated assay for the determination of asparaginase activity was developed. It reproduces exactly the same assay conditions each time it is run. The procedure is fast, precise, accurate, and has eliminated technician to technician variability.
ISSN:0092-1157
DOI:10.1016/S0092-1157(82)80005-X