A photo-CIDNP study of the active sites of Megasphaera elsdenii and Clostridium MP flavodoxins

Megasphaera elsdenii and Clostridium MP flavodoxins have been investigated by photo-CIDNP techniques. Using time-resolved spectroscopy and external dyes carrying different charges it was possible to assign unambiguously the resonance lines in the NMR-spectra to tyrosine, tryptophan and methionine re...

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Veröffentlicht in:	FEBS letters 1982-11, Vol.149 (1), p.141-146
Hauptverfasser:	Moonen, Chrit T.W., Hore, Peter J., Müller, Franz, Kaptein, Robert, Mayhew, Stephen G.
Format:	Artikel
Sprache:	eng
Schlagworte:	3-N-carboxymethyllumiflavin 3-N-ethylaminolumiflavin Binding Sites Clostridium Clostridium - metabolism FID flavin I flavin II FLavodoxin Flavodoxin - metabolism Flavoproteins - metabolism free induction decay Light Magnetic Resonance Spectroscopy Megasphaera elsdenii Megasphaera elsdenii Clostridium MP N.M.R NMR nuclear magnetic resonance Photo-CIDNP photochemically induced dynamic nuclear polarization Species Specificity Veillonellaceae - metabolism
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Zusammenfassung:	Megasphaera elsdenii and Clostridium MP flavodoxins have been investigated by photo-CIDNP techniques. Using time-resolved spectroscopy and external dyes carrying different charges it was possible to assign unambiguously the resonance lines in the NMR-spectra to tyrosine, tryptophan and methionine residues in the two proteins. The results show that Trp-91 in M.elsdenii and Trp-90 in Cl.MP flavodoxin are strongly immobilized and placed directly above the benzene subnucleus of the prosthetic group. The data further indicate that the active sites of the two flavodoxins are extremely similar.
ISSN:	0014-5793 1873-3468
DOI:	10.1016/0014-5793(82)81090-9