Brain isozyme of glycogen phosphorylase: immunohistological localization within the central nervous system

An antibody specific for the predicted carboxyterminal sequence of the human brain isozyme of glycogen phosphorylase (alpha-1,4-D-glucan:orthophosphate D-glucosyltransferase, EC 2.4.1.1) was generated to verify the carboxyterminal amino acid sequence of this protein. The isozyme-specific antibody wa...

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Veröffentlicht in:	Brain research 1990-10, Vol.529 (1-2), p.42-49
Hauptverfasser:	Ignacio, P C, Baldwin, B A, Vijayan, V K, Tait, R C, Gorin, F A
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Astrocytes - enzymology Brain - enzymology Cerebellum - enzymology Cerebral Cortex - enzymology Enzyme-Linked Immunosorbent Assay Humans Immunoblotting Immunoenzyme Techniques Isoenzymes - analysis Isoenzymes - immunology Macaca Mammalia Nerve Fibers, Myelinated Phosphorylases - analysis Phosphorylases - immunology Rats
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Zusammenfassung:	An antibody specific for the predicted carboxyterminal sequence of the human brain isozyme of glycogen phosphorylase (alpha-1,4-D-glucan:orthophosphate D-glucosyltransferase, EC 2.4.1.1) was generated to verify the carboxyterminal amino acid sequence of this protein. The isozyme-specific antibody was used to examine the localization of this protein in primate and non-primate brain. The highest levels of the brain isozyme in cerebrum and cerebellum were found in fibrous astrocytes, many with glial processes that appear to terminate upon blood vessels.
ISSN:	0006-8993
DOI:	10.1016/0006-8993(90)90809-P