DNA-dependent ATPase B of FM3A cells: Its separation from DNA polymerase α

One form of DNA-dependent ATPase (DNA-dependent ATPase B) has been purified from FM3A cells. In this report, we describe the association of DNA polymerase α activity with DNA-dependent ATPase B through a series of purification steps and the final separation of the two enzymes by glycerol gradient ce...

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Veröffentlicht in:	FEBS letters 1982-11, Vol.149 (1), p.44-46
Hauptverfasser:	Watanabe, Yoshinari, Nagata, Kyosuke, Tawaragi, Yasunori, Enomoto, Takemi, Hanaoka, Fumio, Yamada, Masa-atsu
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenosine Triphosphatases - isolation & purification Adenosine Triphosphatases - metabolism Animals Cell Line DNA Helicases DNA Polymerase II - isolation & purification DNA Polymerase II - metabolism DNA polymerase α DNA-dependent ATPase B DNA-Directed DNA Polymerase - isolation & purification FM3A cells Glycerol gradient centrifugation Low salt condition Mice Separation
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Zusammenfassung:	One form of DNA-dependent ATPase (DNA-dependent ATPase B) has been purified from FM3A cells. In this report, we describe the association of DNA polymerase α activity with DNA-dependent ATPase B through a series of purification steps and the final separation of the two enzymes by glycerol gradient centrifugation operated at a low salt concentration.
ISSN:	0014-5793 1873-3468
DOI:	10.1016/0014-5793(82)81067-3