Phosphorylation—dephosphorylation of purified insulin receptor from human placenta: Effect of insulin

Phosphorylation—dephosphorylation of purified insulin receptor from human placenta: Effect of insulin

The insulin receptor of human placenta even after extensive purification is phosphorylated in the presence of [γ- 32P]ATP and NaF, and is dephosphorylated again on incubation in NaF-free medium. Insulin stimulates phosphate incorporation into the M r95 000 subunit probably by activation of the phosp...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:FEBS letters 1982-11, Vol.149 (1), p.96-100
Hauptverfasser: Machicao, F., Urumow, T., Wieland, O.H.
Format: Artikel
Sprache:eng
Schlagworte:
Female
Humans
insulin
Insulin - analogs & derivatives
Insulin - metabolism
Insulin - pharmacology
Insulin action
Insulin receptor
Kinetics
man
Molecular Weight
Phosphorus Radioisotopes
Phosphorylation
Placenta
Placenta - metabolism
Pregnancy
Receptor, Insulin - drug effects
Receptor, Insulin - metabolism
Receptors, Estrogen - isolation & purification
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:The insulin receptor of human placenta even after extensive purification is phosphorylated in the presence of [γ- 32P]ATP and NaF, and is dephosphorylated again on incubation in NaF-free medium. Insulin stimulates phosphate incorporation into the M r95 000 subunit probably by activation of the phosphorylation step. Our data suggest that the insulin receptor contains both kinase and phosphatase activities that may control the phosphorylation state of the receptor.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(82)81079-X