The measurement of human erythrocyte carbonic anhydrase I by the enzyme linked immunosorbent assay (ELISA)

The measurement of human erythrocyte carbonic anhydrase I by the enzyme linked immunosorbent assay (ELISA)

A method is described for the assay of human erythrocyte (RBC) carbonic anhydrase I (HCAI) by the enzyme linked immunosorbent assay. The method was found to be a simple, reliable and precise technique and gave a mean C.V. for 40 samples, assayed in quadruplicate, of 3.32% and a range of 0.84–5.9. Th...

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Veröffentlicht in:Clinical biochemistry 1982-10, Vol.15 (5), p.248-251
Hauptverfasser: Shepherd, Jean N., Spencer, N., Bidwell, D.E., Voller, A.
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Carbonic Anhydrases - blood
Chemical Phenomena
Chemistry
Edetic Acid
Enzyme-Linked Immunosorbent Assay
Erythrocytes - enzymology
Female
Heparin
Humans
Male
Rabbits
Reference Values
Sex Factors
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Zusammenfassung:A method is described for the assay of human erythrocyte (RBC) carbonic anhydrase I (HCAI) by the enzyme linked immunosorbent assay. The method was found to be a simple, reliable and precise technique and gave a mean C.V. for 40 samples, assayed in quadruplicate, of 3.32% and a range of 0.84–5.9. The mean erythrocyte HCAI value and standard deviation for 20 normal men and women were respectively 16.9 ± 3.4 and 15.4 ± 2.1 mg HCAI/g haemoglobin. The use of heparin as an anticoagulant interfered with the assay resulting in apparent HCAI concentrations as low as 60% of those obtained using EDTA.
ISSN:0009-9120
1873-2933
DOI:10.1016/S0009-9120(82)97019-9