A kinetic assay for cellulases

A kinetic assay for cellulases

Research on the mechanism of action of cellulases has been hampered by the lack of a rapid, continuous, or kinetic assay. A linked assay system that uses glucose oxidase and horseradish peroxidase has been coupled with β-glucosidase to yield an assay system that can be used for kinetic assays for ce...

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Veröffentlicht in:Analytical biochemistry 1982-10, Vol.126 (1), p.205-207
Hauptverfasser: Day, D.F., Workman, W.E.
Format: Artikel
Sprache:eng
Schlagworte:
beta-Glucosidase - analysis
Carboxymethylcellulose Sodium - metabolism
Cellobiose - metabolism
Cellulase - analysis
Glucose - analysis
Glucosidases - analysis
Methods
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Zusammenfassung:Research on the mechanism of action of cellulases has been hampered by the lack of a rapid, continuous, or kinetic assay. A linked assay system that uses glucose oxidase and horseradish peroxidase has been coupled with β-glucosidase to yield an assay system that can be used for kinetic assays for cellobiase-producing enzymes as well as a measure of cellobiose degradation by β-glucosidases. This assay shows a 20-fold increase in sensitivity over the traditional reducing sugar assay.
ISSN:0003-2697
1096-0309
DOI:10.1016/0003-2697(82)90130-0