Separation of glycoprotein-N-glycans by high-pH anion-exchange chromatography

A series of high‐mannose and complex type glycoprotein‐N‐glycans was subjected to high‐pH anion‐exchange chromatography. The results revealed that this method represents a useful tool for analytical characterization of single oligosaccharides as well as preparative separation of complex mixtures of carbohydrate side‐chains. On the other hand, it became evident that in several cases a combination of different chromatographic techniques is required for efficient separation of individual oligosaccharide species.