Light and electron microscopic analysis of projection neurons retrogradely labeled with Fluoro-Gold: notes on the application of antibodies to Fluoro-Gold

Fluoro-Gold has been a very popular fluorescent tracer used recently for retrogradely labeling projection neurons. In this study we describe the advantages of using antibodies to Fluoro-Gold in conventional immunohistochemical reaction protocols to further extend the usefulness of this tracer for bo...

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Veröffentlicht in:	Journal of neuroscience methods 1990, Vol.35 (1), p.31-37
Hauptverfasser:	Chang, H.T., Kuo, H., Whittaker, J.A., Cooper, N.G.F.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Anti-Fluoro-Gold Axonal Transport Biological and medical sciences Brain - anatomy & histology Brain - cytology Brain - enzymology Choline O-Acetyltransferase - analysis Fluorescent Antibody Technique Fluorescent Dyes Fluoro-Gold Fundamental and applied biological sciences. Psychology General aspects. Models. Methods Immunoenzyme Techniques Immunohistochemistry - methods Microscopy, Electron - methods Neuroanatomical tracing technique Neurons - cytology Neurons - enzymology Stilbamidines Vertebrates: nervous system and sense organs
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Beschreibung
Zusammenfassung:	Fluoro-Gold has been a very popular fluorescent tracer used recently for retrogradely labeling projection neurons. In this study we describe the advantages of using antibodies to Fluoro-Gold in conventional immunohistochemical reaction protocols to further extend the usefulness of this tracer for both light and electron microscopic neuroanatomical studies.
ISSN:	0165-0270 1872-678X
DOI:	10.1016/0165-0270(90)90091-S