Relationship between DNA chain growth termination and replicon sizes in γ-irradiated mouse cells

Unirradiated mouse leukemic L5178Y cells were pulse-labeled with [ 3H]thymidine continuously for up to 150 minutes. The resulting DNA fiber autoradiograms showed a continuously increasing fiber length up to 70 μm. However, in cells irradiated with 500 to 2000 rad of γ-rays, the resulting DNA fiber l...

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Veröffentlicht in:Journal of molecular biology 1982-10, Vol.160 (4), p.571-578
Hauptverfasser: Sawada, S., Enomoto, T., Kapp, L.N.
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Enomoto, T.
Kapp, L.N.
description Unirradiated mouse leukemic L5178Y cells were pulse-labeled with [ 3H]thymidine continuously for up to 150 minutes. The resulting DNA fiber autoradiograms showed a continuously increasing fiber length up to 70 μm. However, in cells irradiated with 500 to 2000 rad of γ-rays, the resulting DNA fiber lengths leveled off at approximately 35 μm. Independent experiments to estimate replicon size by measuring the center-to-center distance in tandem tracks yielded a value of 48 μm. These results are consistent with a model proposing that the average replicon size is equal to √2 times the plateau value of the average length of labeled DNA measured in irradiated cells.
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subjects Animals
Autoradiography
Cells, Cultured
DNA, Neoplasm - biosynthesis
Gamma Rays
Leukemia L5178 - genetics
Leukemia L5178 - metabolism
Leukemia, Experimental - metabolism
Mice
Replicon - radiation effects
Thymidine - metabolism
Time Factors
title Relationship between DNA chain growth termination and replicon sizes in γ-irradiated mouse cells
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