Glucose Metabolism of Isolated Glomeruli of Rats with Masugi Nephritis

Renal glomeruli were isolated from the rats 4 days after injection of nephrotoxic serum. In an early stage of Masugi nephritis, decarboxylation of 1-14C and 6-14C-glucose, LDH, G6PDH and 6PGDH activities and glycolysis were investigated in the isolated glomeruli. The ratio of 14C-1/14C-6 liberated from labeled glucose was 4.13±0.60 in nephritic glomeruli, while 1.72±0.21 in normal glomeruli. All the enzyme activities mentioned above were significantly increased and glycolysis was accelerated in nephrotic glomeruli. These results indicated that both glycolysic pathway and pentose monophosphate pathway were activated in glomeruli of the rats with Masugi nephritis, and these phenomena may be responsible for the mechanism of repairment in damaged glomeruli.