PREPARATION OF HIGHLY PURIFIED CEPHAMYCIN C

Preparation of highly purified cephamycin C (CM-C) is described in this report. The purification of CM-C was carried out by Sephadex LH-20 chromatography of the N-BOC-CM-C developed with Me0H followed by removal of the protecting group. Partially purified CM-C was further refined by Sephadex LH-20 c...

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Veröffentlicht in:	Japanese journal of antibiotics 1982/07/25, Vol.35(7), pp.1699-1700
Hauptverfasser:	KUNISHIMA, MAMORU, YABUUCHI, MASAHIKO, MASUDA, MINORU, IMAZUMI, KIKUO, HAMANO, HIROAKI
Format:	Artikel
Sprache:	jpn
Schlagworte:	Cephalosporins - isolation & purification Cephamycins - isolation & purification Methods
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Zusammenfassung:	Preparation of highly purified cephamycin C (CM-C) is described in this report. The purification of CM-C was carried out by Sephadex LH-20 chromatography of the N-BOC-CM-C developed with Me0H followed by removal of the protecting group. Partially purified CM-C was further refined by Sephadex LH-20 chromatography developed with Me0H and Diaion HP-20 SS chromatography developed with H2O. CM-C thus obtained showed the UV absorption at 265 nm [E1%1cm 184.4 (H2O) 1] which is the strongest absorption ever appeared in the literatures.
ISSN:	0368-2781 2186-5477
DOI:	10.11553/antibiotics1968b.35.1699