Gene mapping of mouse laminin A and B2 subunits using mouse-Chinese hamster somatic cell hybrids

Gene mapping of mouse laminin A and B2 subunits using mouse-Chinese hamster somatic cell hybrids

Laminin is a multichain extracellular matrix glycoprotein found primarily in basement membranes. The molecule is made up of three subunits, designated as A, B1, and B2. Using an 850-base cDNA probe for the mouse laminin A-chain and a 1000-base cDNA probe for the mouse laminin B2 chain, we have scree...

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Veröffentlicht in:Somatic cell and molecular genetics 1990-11, Vol.16 (6), p.599-603
Hauptverfasser: WEBER KAYE, N, CHUNG, A. E, LALLEY, P. A, DURKIN, M. E, PHILLIPS, S. L, CHURCH, R. L
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Biological and medical sciences
Blotting, Southern
Classical genetics, quantitative genetics, hybrids
Fundamental and applied biological sciences. Psychology
Genetics of eukaryotes. Biological and molecular evolution
Hybrid Cells
Laminin - genetics
Mice
Vertebrata
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Zusammenfassung:Laminin is a multichain extracellular matrix glycoprotein found primarily in basement membranes. The molecule is made up of three subunits, designated as A, B1, and B2. Using an 850-base cDNA probe for the mouse laminin A-chain and a 1000-base cDNA probe for the mouse laminin B2 chain, we have screened mouse-Chinese hamster somatic cell hybrids in order to assign the genes for each of these polypeptides to their respective mouse chromosome. We have determined that the mouse laminin B2-chain gene is located on chromosome 1 (confirming this assignment) and the laminin A-chain gene is located on chromosome 17.
ISSN:0740-7750
1572-9931
DOI:10.1007/BF01233101