STUDIES OF HETEROTRANSPLANTATION OF TRANSPLANTABLE HUMAN UROGENITAL MALIGNANT NEOPLASMS: Part V. Chemotherapy against the Carcinoma of Human Urinary Bladder Transplantable to the Nude Mice (NM-B-1)

The sensitivity of 12 anti-cancer drugs against the carcinoma of human urinary bladder transplantable to nude mice (NM-B-1) were tested. NM-B-1 was poorly differentiated transitional cell carcinoma with partial squamous metaplasia, and its successful transplantation rate was almost 100%. Its 15th to 18th passages were used for this study, and their growth rate were similar and stable. The effects of drugs were evaluated by three means such as tumor growth curve, light and electron microscopic findings. Drugs applied in this study were as follows: CDDP, IFM, CTX, BLM, PLM, ADM, MMC, MTX, ACNU, 5-FU, VLB, VP-16. All drugs administered to nude mice intraperitoneally. The results from the tumor growth curve, CDDP 2.5, 5mg/kg, IFM 100mg/kg, BLM 20, 40mg/kg, PLM 1.5, 3, 6mg/kg were effective as single agents. All drugs such as BLM, PLM, ADM, IFM, ADM+ IFM combined with CDDP 2.5mg/kg were effecacious. The combination of ADM 4mg/kg+IFM100mg/kg was also effective. Light microscopic examination revealed that squamous cell pattern was dominant when CDDP single administration or combined with PLM. On the contrary, transitional cell pattern was prevailing when other drugs were applied. Electron microscopic investigation revealed an increase of intracellular vacuolation and tonofibrils, and shortening or disappearance of microvilli when CDDP singly or combination of CDDP+PLM, ADM+IFM as administered. These findings were thought to be a reduction of cytoviability. From the above results, it is concluded that the combination of CDDP 2.5mg/kg+PLM 3mg/kg is most effective to NM-B-1.