Effect of prostaglandin E2 on cyclic AMP levels in limb cells of mouse mutant Brachypodism

Mouse embryo limb cells carrying either the brachypodism (bpH/bpH) mutation or its wild-type (+/+) allele were tested for their ability to accumulate cyclic AMP in response to prostaglandin E2 (PGE2) between Embryonic Days E12 and E14. Mutant cells exhibited a precocious increase in cyclic AMP. In t...

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Veröffentlicht in:	Developmental biology 1990-12, Vol.142 (2), p.489-492
Hauptverfasser:	BALLARD, T. A, ELMER, W. A
Format:	Artikel
Sprache:	eng
Schlagworte:	1-Methyl-3-isobutylxanthine - pharmacology Alleles Animals Biological and medical sciences Blastocyst - drug effects Blastocyst - metabolism Cell Differentiation Cyclic AMP - metabolism Dinoprostone - pharmacology Embryology: invertebrates and vertebrates. Teratology Experimental organogenesis Fundamental and applied biological sciences. Psychology Hindlimb - drug effects Hindlimb - embryology Hindlimb - metabolism Mice Mutation Organogenesis. Physiological fonctions
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Zusammenfassung:	Mouse embryo limb cells carrying either the brachypodism (bpH/bpH) mutation or its wild-type (+/+) allele were tested for their ability to accumulate cyclic AMP in response to prostaglandin E2 (PGE2) between Embryonic Days E12 and E14. Mutant cells exhibited a precocious increase in cyclic AMP. In the absence of PGE2 but in the presence of the phosphodiesterase inhibitor 1-methyl-3-isobutylxanthine (MIX), the brachypodism cells accumulated a significantly lower amount of cyclic AMP by Day E14. Limb cells carrying the bpH mutation may provide a useful experimental system to study the PGE2-cyclic AMP-cartilage differentiation interrelationship.
ISSN:	0012-1606 1095-564X
DOI:	10.1016/0012-1606(90)90372-P