R-banding and nonisotopic in situ hybridization : precise localization of the human type II collagen gene (COL2A1)

A new mapping system, based on nonisotopic in situ hybridization combined with fluorescent staining of replicated prometaphase R-bands, is described. Replication of the bands is achieved by treatment of thymidine-synchronized cells with bromodeoxyuridine. The human COL2A1 gene was mapped to band 12q...

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Veröffentlicht in:	Human genetics 1990-11, Vol.86 (1), p.14-16
Hauptverfasser:	TAKAHASHI, E.-I, HORI, T.-A, O'CONNELL, P, LEPPERT, M, WHITE, R
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Chromosome Banding - methods Chromosome Mapping - methods Chromosomes, Human, Pair 12 Classical genetics, quantitative genetics, hybrids Collagen - genetics DNA Probes Fundamental and applied biological sciences. Psychology Genetics of eukaryotes. Biological and molecular evolution Human Humans Karyotyping Nucleic Acid Hybridization
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Beschreibung
Zusammenfassung:	A new mapping system, based on nonisotopic in situ hybridization combined with fluorescent staining of replicated prometaphase R-bands, is described. Replication of the bands is achieved by treatment of thymidine-synchronized cells with bromodeoxyuridine. The human COL2A1 gene was mapped to band 12q13.11-q13.12 in this manner, to illustrate the potential of the technique for improving the precision of chromosomal mapping and physical ordering of genes.
ISSN:	0340-6717 1432-1203
DOI:	10.1007/bf00205165