Structural Basis of Transcription: Separation of RNA from DNA by RNA Polymerase II

The structure of an RNA polymerase II-transcribing complex has been determined in the posttranslocation state, with a vacancy at the growing end of the RNA-DNA hybrid helix. At the opposite end of the hybrid helix, the RNA separates from the template DNA. This separation of nucleic acid strands is b...

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Veröffentlicht in:	Science (American Association for the Advancement of Science) 2004-02, Vol.303 (5660), p.1014-1016
Hauptverfasser:	Westover, Kenneth D., Bushnell, David A., Kornberg, Roger D.
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Pairing Biological and medical sciences Chemical bases Chemical properties Coding Crystalline structure Crystallization Crystallography, X-Ray Deoxyribonucleic acid DNA DNA mapping DNA, Single-Stranded - chemistry DNA, Single-Stranded - metabolism Electron density Fundamental and applied biological sciences. Psychology Genetic transcription Hybridity Models, Molecular Molecular and cellular biology Molecular biophysics Molecular genetics Nucleic Acid Conformation Nucleic Acid Hybridization Nucleic acids Nucleotides Oligodeoxyribonucleotides - chemistry Oligodeoxyribonucleotides - metabolism Oligonucleotides Oligoribonucleotides - chemistry Oligoribonucleotides - metabolism Promoter Regions, Genetic Protein Conformation Proteins Ribonucleic acid RNA RNA polymerase RNA Polymerase II - chemistry RNA Polymerase II - metabolism RNA polymerases RNA, Complementary - chemistry RNA, Complementary - metabolism Saccharomyces cerevisiae - enzymology Structure in molecular biology Templates, Genetic Transcription (Genetics) Transcription Factor TFIIB - metabolism Transcription, Genetic Transcription. Transcription factor. Splicing. Rna processing Transcripts (Written Records)
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Zusammenfassung:	The structure of an RNA polymerase II-transcribing complex has been determined in the posttranslocation state, with a vacancy at the growing end of the RNA-DNA hybrid helix. At the opposite end of the hybrid helix, the RNA separates from the template DNA. This separation of nucleic acid strands is brought about by interaction with a set of proteins loops in a strand/loop network. Formation of the network must occur in the transition from abortive initiation to promoter escape.
ISSN:	0036-8075 1095-9203
DOI:	10.1126/science.1090839