A comparison of the initiating abilities of ribo- and deoxyriboprimers in DNA polymerization catalyzed by AMV reverse transcriptase

The difference in optimal conditions for DNA polymerization catalyzed by AMV reverse transcriplase on poly(A) and poly(dA) templates with d(pT) 10 and (pU) 10 primers has been found. A comparison of the initiating abilities of d(pT) 10 and (pU) 10, primers under optimal conditions for various templa...

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Veröffentlicht in:	FEBS letters 1990-11, Vol.274 (1), p.156-158
Hauptverfasser:	Lokhova, I.A., Nevinsky, G.A., Gorn, V.V., Veniaminova, A.G., Repkova, M.V., Kavsan, V.M., Rudenko, N.K., Lavrik, O.I.
Format:	Artikel
Sprache:	eng
Schlagworte:	AMV reverse transcriptase avian myeloblastosis virus Avian Myeloblastosis Virus - enzymology Biological and medical sciences Cell physiology DNA Replication Fundamental and applied biological sciences. Psychology Kinetics Km for primer Molecular and cellular biology Polynucleotides RNA-Directed DNA Polymerase - metabolism Structure-Activity Relationship Templates, Genetic
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Zusammenfassung:	The difference in optimal conditions for DNA polymerization catalyzed by AMV reverse transcriplase on poly(A) and poly(dA) templates with d(pT) 10 and (pU) 10 primers has been found. A comparison of the initiating abilities of d(pT) 10 and (pU) 10, primers under optimal conditions for various template · primer complexes has been made. The best template · primer complex was poly(A) · d(pT) 10 and the worst was poly(A) · (pU) 10. The lengthening of d (pT) n primers by a mononucleotide unit ( n=2 10) increases their affinity by a factor of about 2 and 3 in the case of poly(dA) and poly(A) templates, respectively. The affinities ofd (pT) 11–25 to the enzyme does not change with the primer length.
ISSN:	0014-5793 1873-3468
DOI:	10.1016/0014-5793(90)81352-O