The genome of Haemophilus influenzae Rd has a unique NotI site

Previous analysis of physical maps of Haemophilus influenzae, which is circular and 1.9 Mb in length [Lee and Smith, J. Bacteriol. 170 (1988) 4402–4405; Kauc et al., J. Bacteriol. 171 (1989) 2474–2479], did not detect any NotI (GCGGCCGC) restriction sites. A transposon, Tn 916, was constructed to co...

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Veröffentlicht in:	Gene 1990-10, Vol.95 (1), p.149-153
Hauptverfasser:	Kauc, Leszek, Mitchell, Marilyn A., Goodgal, Sol H.
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence Biological and medical sciences Blotting, Southern Cloning, Molecular Deoxyribonucleases, Type II Site-Specific - genetics DNA Transposable Elements DNA, Circular - analysis field-inversion gel electrophoresis Fundamental and applied biological sciences. Psychology Genes, Viral Genes. Genome Haemophilus influenzae - genetics Haemophilus sp Molecular and cellular biology Molecular genetics Molecular Sequence Data Polymorphism, Restriction Fragment Length Recombinant DNA restriction enzyme mapping Restriction Mapping Tn 916 transposition
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Zusammenfassung:	Previous analysis of physical maps of Haemophilus influenzae, which is circular and 1.9 Mb in length [Lee and Smith, J. Bacteriol. 170 (1988) 4402–4405; Kauc et al., J. Bacteriol. 171 (1989) 2474–2479], did not detect any NotI (GCGGCCGC) restriction sites. A transposon, Tn 916, was constructed to contain a NotI linker cloned into its NciI site and introduced into the H. influenzae chromosome. NotI digestion of chromosomes containing a Tn 916-associated NotI site followed by separation of fragments by field-inversion gel electrophoresis revealed the presence of two fragments obtained by two NotI cuts, one in Tn 916 and the other, a unique, ‘natural’ NotI site in the original chromosomal DNA. The examination of other Haemophilus strains demonstrated the presence of one or more NotI sites in all of those tested.
ISSN:	0378-1119 1879-0038
DOI:	10.1016/0378-1119(90)90427-S