An improved method for the detection of Dcm methylation in DNA molecules

The intrinsic insensitivity of EcoRII recognition sites in RF DNAs of phage M13 and vector M13mp18 towards this restriction endonuclease can be overcome by adding site-specific oligodeoxyribonucleotide duplexes to the restriction sample. Since Dcm − DNA but not Dcm +-methylated DNA becomes susceptib...

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Veröffentlicht in:	Gene 1990-10, Vol.95 (1), p.161-162
Hauptverfasser:	Reuter, Monika, Pein, Claus-Dietmar, Butkus, Viktoras, Krüger, Detlev H.
Format:	Artikel
Sprache:	eng
Schlagworte:	bacteriophage M13 Bacteriophages - genetics Biological and medical sciences Cytosine DNA - analysis DNA methylation Electrophoresis, Agar Gel Fundamental and applied biological sciences. Psychology Genes. Genome Methylation Molecular and cellular biology Molecular genetics oligodeoxyribonucleotide duplexes Polymorphism, Restriction Fragment Length restriction endonuclease BstNI restriction endonuclease EcoRII restriction endonuclease MvaI vector M13mp18
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Beschreibung
Zusammenfassung:	The intrinsic insensitivity of EcoRII recognition sites in RF DNAs of phage M13 and vector M13mp18 towards this restriction endonuclease can be overcome by adding site-specific oligodeoxyribonucleotide duplexes to the restriction sample. Since Dcm − DNA but not Dcm +-methylated DNA becomes susceptible under these conditions, this procedure constitutes an improvement of the Dcm methylation assay.
ISSN:	0378-1119 1879-0038
DOI:	10.1016/0378-1119(90)90429-U