Expression of mammalian renal transporters in Xenopus laevis oocytes

We have injected mRNA from rabbit renal cortex into Xenopus oocytes and demonstrated the expression of renal carriers for Na +-independent transport of l-phenylalanine and l-lysine and Na +-dependent transport of l-alanine and succinate. Maximal expression of renal amino acid transporters occurred 6...

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Veröffentlicht in:	Archives of biochemistry and biophysics 1990-11, Vol.283 (1), p.130-134
Hauptverfasser:	Coady, Michael J., Pajor, Ana M., Toloza, Eric M., Wright, Ernest M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Transport Systems Amino Acids - metabolism Animals Biological and medical sciences Biological Transport - drug effects Carrier Proteins - genetics Carrier Proteins - metabolism Female Fundamental and applied biological sciences. Psychology Gene expression Kidney Cortex - physiology Kinetics Microinjections Molecular and cellular biology Molecular genetics Molecular Weight Oocytes - physiology Rabbits RNA, Messenger - administration & dosage RNA, Messenger - genetics RNA, Messenger - isolation & purification Sodium - pharmacology Xenopus laevis
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Zusammenfassung:	We have injected mRNA from rabbit renal cortex into Xenopus oocytes and demonstrated the expression of renal carriers for Na +-independent transport of l-phenylalanine and l-lysine and Na +-dependent transport of l-alanine and succinate. Maximal expression of renal amino acid transporters occurred 6–8 days following mRNA injection. The proteins responsible for transport of these four substrates were translated from mRNAs which are between 1.5 and 3.0 kb. This information serves as a starting point for expression cloning of these transport proteins.
ISSN:	0003-9861 1096-0384
DOI:	10.1016/0003-9861(90)90622-6