Thioglycosynthases: double mutant glycosidases that serve as scaffolds for thioglycoside synthesis
A double mutant, retaining glycosidase that lacks both the catalytic nucleophile and the catalytic acid/base residues efficiently catalyzes thioglycoside formation from a glycosyl fluoride donor and thiosugar acceptors.
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| Veröffentlicht in: | Chemical communications (Cambridge, England) England), 2004-02 (3), p.274-275 |
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| container_end_page | 275 |
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| container_issue | 3 |
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| container_title | Chemical communications (Cambridge, England) |
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| creator | Jahn, Michael Chen, Hongming Müllegger, Johannes Marles, Jennifer Warren, R Antony J Withers, Stephen G |
| description | A double mutant, retaining glycosidase that lacks both the catalytic nucleophile and the catalytic acid/base residues efficiently catalyzes thioglycoside formation from a glycosyl fluoride donor and thiosugar acceptors. |
| doi_str_mv | 10.1039/b313155f |
| format | Article |
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| fulltext | fulltext |
| identifier | ISSN: 1359-7345 |
| ispartof | Chemical communications (Cambridge, England), 2004-02 (3), p.274-275 |
| issn | 1359-7345 1364-548X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_80109351 |
| source | MEDLINE; Royal Society of Chemistry Journals Archive (1841-2007); Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
| subjects | Circular Dichroism Enzyme Stability Glycoside Hydrolases - chemistry Glycoside Hydrolases - genetics Glycoside Hydrolases - metabolism Hydrogen-Ion Concentration Molecular Structure Mutation - genetics Temperature Thioglycosides - biosynthesis Thioglycosides - chemistry |
| title | Thioglycosynthases: double mutant glycosidases that serve as scaffolds for thioglycoside synthesis |
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