Thioglycosynthases: double mutant glycosidases that serve as scaffolds for thioglycoside synthesis

Thioglycosynthases: double mutant glycosidases that serve as scaffolds for thioglycoside synthesis

A double mutant, retaining glycosidase that lacks both the catalytic nucleophile and the catalytic acid/base residues efficiently catalyzes thioglycoside formation from a glycosyl fluoride donor and thiosugar acceptors.

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Bibliographische Detailangaben
Veröffentlicht in:Chemical communications (Cambridge, England) England), 2004-02 (3), p.274-275
Hauptverfasser: Jahn, Michael, Chen, Hongming, Müllegger, Johannes, Marles, Jennifer, Warren, R Antony J, Withers, Stephen G
Format: Artikel
Sprache:eng
Schlagworte:
Circular Dichroism
Enzyme Stability
Glycoside Hydrolases - chemistry
Glycoside Hydrolases - genetics
Glycoside Hydrolases - metabolism
Hydrogen-Ion Concentration
Molecular Structure
Mutation - genetics
Temperature
Thioglycosides - biosynthesis
Thioglycosides - chemistry
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Beschreibung
Zusammenfassung:A double mutant, retaining glycosidase that lacks both the catalytic nucleophile and the catalytic acid/base residues efficiently catalyzes thioglycoside formation from a glycosyl fluoride donor and thiosugar acceptors.
ISSN:1359-7345
1364-548X
DOI:10.1039/b313155f