A new peptide in the FMRFamide family isolated from the CNS of the hawkmoth, Manduca sexta

A new peptide in the FMRFamide family isolated from the CNS of the hawkmoth, Manduca sexta

We have purified a FMRFamide-like peptide from extracts of brain-subesophageal ganglion of the moth, Manduca sexta. The purification was monitored with a new, competitive ELISA, and accomplished with ion exchange and reverse-phase HPLC. The peptide structure was determined by a combination of tandem...

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Veröffentlicht in:Peptides (New York, N.Y. : 1980) N.Y. : 1980), 1990-07, Vol.11 (4), p.849-856
Hauptverfasser: Kingan, Timothy G., Teplow, David B., Phillips, Jessica M., Riehm, John P., Ranga Rao, K., Hildebrand, John G., Homberg, Uwe, Kammer, Ann E., Jardine, Ian, Griffin, Patrick R., Hunt, Donald F.
Format: Artikel
Sprache:eng
Schlagworte:
Amino Acid Sequence
Animals
central nervous system
Central Nervous System - chemistry
Enzyme-Linked Immunosorbent Assay
flight
FMRFamide
FMRFamide family
Hawkmoth
HPLC
Immunoassay
Ion exchange
Lepidoptera
Manduca sexta
Molecular Sequence Data
Moths - analysis
Neuropeptides - chemistry
Neuropeptides - isolation & purification
Neuropeptides - physiology
Peptide purification
Sphingidae
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Zusammenfassung:We have purified a FMRFamide-like peptide from extracts of brain-subesophageal ganglion of the moth, Manduca sexta. The purification was monitored with a new, competitive ELISA, and accomplished with ion exchange and reverse-phase HPLC. The peptide structure was determined by a combination of tandem mass spectrometry and automated Edman degradation. The amino acid sequence of the peptide is
ISSN:0196-9781
1873-5169
DOI:10.1016/0196-9781(90)90203-H