PCR-generated artificial ribosomal DNAs from premature termination at Alu sequences

PCR-amplified product may sometimes not correlate with a DNA state in vivo due to formation of recombinant molecules. Here we show that recombinant product can form in vitro on amplifying the region upstream of the rRNA transcription start point in human ribosomal intergenic spacer. These results pr...

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Veröffentlicht in:	Biomolecular engineering 2004, Vol.21 (1), p.21-25
Hauptverfasser:	Kupriyanova, N.S., Shibalev, D.V., Voronov, A.S., Ryskov, A.P.
Format:	Artikel
Sprache:	eng
Schlagworte:	Alu Elements - genetics Alu repeats Base Sequence Codon, Nonsense - genetics DNA, Ribosomal Spacer - genetics Gene Expression Regulation - genetics Genetic Variation Human rIGS Humans Microsatellites Midisatellites Molecular Sequence Data PCR amplification PCR recombinant product Polymerase Chain Reaction - methods Premature polymerase termination Recombination, Genetic - genetics RNA, Ribosomal - genetics Sequence Homology, Nucleic Acid
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Beschreibung
Zusammenfassung:	PCR-amplified product may sometimes not correlate with a DNA state in vivo due to formation of recombinant molecules. Here we show that recombinant product can form in vitro on amplifying the region upstream of the rRNA transcription start point in human ribosomal intergenic spacer. These results provide the first information concerning definite Alu sites where premature polymerase termination occurs.
ISSN:	1389-0344 1878-559X
DOI:	10.1016/S1389-0344(03)00004-2